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. 2016 Jun 13;6:27734. doi: 10.1038/srep27734

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Copyright © 2016, Macmillan Publishers Limited

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Optical micrograph of the decellularized cornea stained with toluidine blue at 8 weeks after interlamellar keratoplasty (a). The TEM images of the indicated squares in a are shown in (b–k). The dotted line region indicates the transplanted decellularized cornea. TEM image of receptive rabbit cornea showing two preferred orientations of collagen fibrils in successive lamella structure (b,c). Granulocyte was observed around the transplanted decellularized cornea (d,e). TEM observation revealed that the structure of the collagen fibrils was more randomly organized in the inside (f– i) than in the periphery (j,k) of the decellularized cornea. Scale bars: 100 μm in (a); 1 μm in (b,d,f,h,j); and, 3 μm in (c,e,g,i,k).