Table 1. Selection and targeting efficiencies in the CRISPR/Cas9 mutagenic screens.

Gene	Type		Amounts of sgRNA (Cas9 protein)
			Control	10 μg (7.5 μg)	20 μg (15 μg)	40 μg (30 μg)	Total
MAA7-1	Selection	Colonies	0	1	2	–a	3
		Efficiency (cell−1)b	0	1.1 × 10−8	6.7 × 10−8	–	3.3 × 10−8
	Targeting	Colonies	0	0	0	–	0 (0%)c
		Efficiency (cell−1)	0	0	0	–	0
MAA7-2	Selection	Colonies	1	6	5	9	20
		Efficiency (cell−1)	1.1 × 10−8	6.7 × 10−8	5.6 × 10−8	1.0 × 10−7	2.2 × 10−7
	Targeting	Colonies	0	0	2	6	8 (40%)
		Efficiency (cell−1)	0	0	2.2 × 10−8	6.7 × 10−8	8.9 × 10−8
CpSRP43	Selection	Colonies	65	34	108	76	218
		Efficiency (cell−1)	7.2 × 10−7	3.8 × 10−7	1.2 × 10−6	8.4 × 10−7	2.4 × 10−6
	Targeting	Colonies	0	3	0	0	3 (1.4%)
		Efficiency (cell−1)	0	3.3 × 10−8	0	0	3.3 × 10−8
ChlM	Selection	Colonies	–	6000	–	–	6000
		Efficiency (cell−1)	–	3.0 × 10−5	–	–	3.0 × 10−5
	Targeting	Colonies	–	10	–	–	10 (0.17%)
		Efficiency (cell−1)	–	5.0 × 10−8	–	–	5.0 × 10−8

^aNot tested.

^bThe efficiency was calculated by dividing the number of colonies with that of cells used for electroporation.

^cTargeting percentage was calculated by dividing the number of targeted colonies with that of selected colonies.