Figure 3.

Age dependence of adventitial LYVE-1⁺ cells. Immunofluorescence staining for lymphatic endothelium (LYVE-1, red) and the leukocyte activation marker, CD11b (green). A) Aortic arch cryosections. Lymph node, positive control. B, C) Quantification of adventitial CD11b⁺ LYVE-1⁻ cells (B) and LYVE-1⁺ CD11b⁺ cells (C) (young WT mice: n = 5; 8–12 wk; M:F, 0.6; aged WT mice: n = 5; 54–58 wk; M:F, 0.6; young apoE^−/− mice: n = 5; 8–12 wk; M:F, 0.6; aged apoE^−/− mice: n = 5; 54–58 wk; M:F, 0.6; WD: n = 3; 32 wk; M:F, 0.6), means ± sem. ^✻P < 0.05, ^✻✻P < 0.01.