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. 2016 Mar 22;30(7):2490–2499. doi: 10.1096/fj.201500112

TABLE 1.

Composition of the semipurified WD

WD composition
Ingredient Amount
CHO/fat/protein (% calories) 60:20:20
Cholesterol (g/kg) 10
Casein (g/kg) 105
Lactalbumin (g/kg) 105
Dextrose (g/kg) 186
Sucrose (g/kg) 186
Cornstarch (g/kg) 194
Margarine B (80% fat) (g/kg) 116 (93 fat)
Mineral mix (g/kg) 46
Vitamin mix (g/kg) 12
Choline chloride (g/kg) 3

Carbohydrate, fat, and protein ratio (60:20:20% calories) represents the percentage of dietary calories provided by each nutrient category. Diet cholesterol was added at 1% by weight, and margarine B was a blend of fats common in the U.S. diet. It was based on saturated animal fat as 24% milk fat and 40% beef tallow, with sources of monounsaturated and polyunsaturated fats from 20% chicken fat and 16% soybean oil. The ratio of saturated to monounsaturated to polyunsaturated fatty acids was approximately 44:38:18. The diet was adjusted for vitamin and mineral concentrations as a percentage of calories (4.2 kcal/g). CHO, carbohydrate; prot, protein.