Figure 4.
Raptor is required for up-regulation of p70S6K by aldosterone. A) Raptor expression was inhibited in cultured PASMCs using si-Raptor (40 nM; n = 3). B) PASMCs transfected with si-Raptor and stimulated with aldosterone (10−7 M) for 1 h demonstrated a decrease in the ratio of P-p70S6K(Thr389)-to-p70S6K compared with aldosterone (ALDO)-stimulated cells treated with vehicle control (V; n = 3) or transfected with si-Scc. Un, untreated; V, lipofectamine transfection reagent. Data are presented as mean ± sem. Representative immunoblots are shown.