Figure 5.

Pharmacologic MR antagonism inhibits aldosterone (ALDO)-induced up-regulation of Akt/Raptor/p70S6K in PASMCs in vitro. Human PASMCs were treated with vehicle control or aldosterone (10⁻⁷ M) for 1 h in the presence or absence of spironolactone (SP; 10 μM), and expression levels of P-Akt(Ser473) (A), P-Raptor(Ser792) (B), and P-p70S6K(Thr389) (C) were measured by immunoblot and compared with levels of the respective unmodified proteins (n = 3 for each blot). V, vehicle. Data are presented as mean ± sem. Representative blots are shown.