Skip to main content
. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Dec 29;292:40–55. doi: 10.1016/j.taap.2015.12.020

Figure 7.

Figure 7

Recovery of ATP in HepaRG cells pretreated with the CYP2E1 inhibitor CMZ and subsequently treated with APAP. HepaRG cells were incubated for 1 week with different conditions of incubation with 100 µM stearic acid (C18:0), 100 µM oleic acid (C18:1), two concentrations of insulin and 150 µM CMZ and subsequently treated or not with 2.5 or 20 mM of APAP. ATP levels were measured 6, 24 or 48 h after APAP treatment. ATP recovery was determined by calculating the difference of ATP levels measured with and without CMZ. Results are means ± SEM for 3 independent cultures, with data in triplicates for each culture. Letters F and I indicate a significant effect (P<0.05) of fatty acids and insulin, respectively and ns, not significant with a two-way ANOVA analysis. *Significantly different from HepaRG cells incubated without fatty acids and with the same condition of insulin treatment (P<0.05). #Significantly different from HepaRG cells incubated without insulin and with the same condition of fatty acid treatment (P<0.05).