(A) 2 μM trametinib was toxic to control flies; toxicity was rescued by 50 μM fluvastatin (Flu; p≤0.05). The proteasome inhibitor bortezomib (bor; p≤0.05) and three mTOR inhibitors—everolimus (eve; p≤0.01), rapamycin (rap; p≤0.05) and temserolimus (tem; p≤0.05)—were also toxic at the specified doses (μM). All were partially rescued by 50 μM fluvastatin.
(B, C) Mutant lines of the Drosophila homologs of HMG-CoA reductase and farnesyl pyrophosphate synthase were insensitive to trametinib and/or everolimus toxicity.
P values are *≤0.05, **≤0.01, ***≤0.01, ****≤0.0001; values represent mean ± SEM.