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. 2016 Jun 13;6:27690. doi: 10.1038/srep27690

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Copyright © 2016, Macmillan Publishers Limited

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Absorbance difference spectra obtained by the progressive titration of 5 μM CYP51 with eburicol (A) and 4 μM CYP5218 with palmitoleic acid (B) were measured. Saturation curves (C) for eburicol (filled circles) and palmitoleic acid (hollow circles) were constructed from the absorbance difference spectra. Ligand binding data were fitted using the Michaelis-Menten equation with each experiment performed in triplicate although only one replicate is shown.