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3D surface profile of polyHEMA coated substrate by (a) conventional evaporation process or (b) by spin coating and reflow process. MDA-MB-231 cells were cultured for 24 hours on (c–e) non-coated glass substrate or (f–h) polyHEMA coated substrate. Phase contrast micrographs (c,f) and scanning electron micrographs (d,g) of single MDA-MB-231 cells. (e,h) MDA-MB-231 cells were stained for actin (red), vinculin (green), and nucleus (DAPI blue).
