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. 2016 Jun 13;6:27301. doi: 10.1038/srep27301

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(a–e) The development of a patient derived xenograft (PDX) sphere from a single cell in the polyHEMA-coated suspension culture micro-chamber: (a) day 0, (b) day 7, (c) day 14, (d) day 21, (e) day 28. (f–h) The development of single-cell derived sphere formation for various cell lines: (f) SUM159 after 10 days, (g) MCF-7 after 10 days, and (h) T47D after 10 days. (i) The growth (diameter) of SUM159, T47D, and MCF-7 spheres from day 0 to day 10. (j) The growth (diameter) of PDX samples over 28-day period. Scale bar: 100 μm.