Table 2. Identification (LC-MS) of proteins in isolated Langerhan’s islets from rats exposed to vehicle or PCB126 (10 μg/kg).
| SwissProt ID | Protein ID | Match ID | PCB/Vehicle ratio | Biological function |
|---|---|---|---|---|
| Response to oxidative stress | ||||
| Q66HD0 | Heat shock protein 90 kDa (HSP90) | 68 | +5.94 | Processing and transport of secreted proteins. |
| P13803 | Electron transfer flavoprotein subunit alpha (Alpha-ETF) | 16 | +3.38 | Electron acceptor for several dehydrogenases; transfer of electrons to mitochondrial respiratory chain. |
| P06761 | 78 kDa glucose-regulated protein (GRP78) | 64 | +1.60 | Folding of proteins and degradation of misfolded proteins. |
| P19804 | Nucleoside diphosphate kinase B (NDK B) | 0 | −2.48 | Synthesis of nucleosides; cellular response to fatty acids, glucose, and oxidative stress. |
| Q05982 | Nucleoside diphosphate kinase A (NDK A) | 0 | −2.48 | Synthesis of nucleosides; cellular response to fatty acids, glucose, and drugs. |
| P29315 | Ribonuclease inhibitor | 42 | −3.46 | Inhibitor of RNAses; may play a role in redox homeostasis. |
| Carbohydrate metabolism | ||||
| Q9ER34 | Aconitate hydratase | 65 | +4.47 | Catalyses the isomerization of citrate to isocitrate. |
| P07943 | Aldose reductase (AR) | 22 | +2.54 | Reduction of carbonyl-containing compounds. |
| P00689 | Pancreatic alpha-amylase | 51 | −1.62 | Hydrolysis of alpha bonds of large alpha-linked polysaccharides. |
| P10760 | Adenosylhomocysteinase | 36 | −2.25 | Regulation of the intracellular concentration of adenosylhomocysteine. |
| Lipid metabolism | ||||
| P1 8886 | Carnitine O-palmitoyltransferase 2 (CPT2) | 61 | +3.30 | Carnitine O-palmitoyltransferase activity; fatty acid oxidation and transport. |
| P17764 | Acetyl-CoA acetyltransferase | 26 | −5.26 | Acetyl-CoA C-acetyltransferase activity; ketone body metabolism. |
| Cytoplasm/cytoskeleton proteins | ||||
| Q6IFW6 | Keratin, type I cytoskeletal 10 | 2 | +2.05 | Belongs to the superfamily of structural proteins which form the intermediate filaments. |
| P60711 | Actin, cytoplasmic 1 | 32 | −1.46 | Cell motility. |
| P63029 | Translationally-controlled tumor protein (TCTP) | 4 | −2.47 | Calcium binding and microtubule stabilization. |
| Digestive enzymes | ||||
| P08426 | Cationic trypsin-3 | 5 | −1.61 | Serine-type endopeptidase activity. |
| P07338 | Chymotrypsinogen B | 12 | −1.72 | Serine-type endopeptidase activity. |
| P27657 | Pancreatic triacylglycerol lipase | 38 | −2.11 | Splits the esters of long-chain fatty acids to produce monoacylglycerol and free fatty acids. |
| P54316 | Inactive pancreatic lipase-related protein 1 | 53 | −2.64 | Inhibitor of dietary triglyceride digestion. |
| Other proteins | ||||
| Q5U2Q3 | Ester hydrolase C11orf54 homolog | 19 | +2.13 | Exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate. |
| P00731 | Carboxypeptidase A1 | 33 | −2.13 | Catalyses the release of a C-terminal amino acid. |
| P10760 | Adenosylhomocysteinase | 36 | −2.25 | Regulation of the intracellular concentration of adenosylhomocysteine. |
SwissProt ID refers to UniProt taxonomy Rattus norvegicus (http://www.uniprot.org/taxonomy/10116). Match ID refers to the pair number in 2D gel (Fig. 5a). Fold change in expression of proteins is presented as PCB126/vehicle ratio; (+) indicates up-regulated protein, (−) indicates down-regulated proteins in islets of Langerhans from rats exposed to PCB126.