Fig. 3.

Presence of phosphoregulation of the penultimate threonine in pT-type OSAs. (A) Western blot showing the specificity of antibodies (anti-CAT and anti-pThr) against protein extracts of rice leaf blades harvested under light. Molecular weights are shown on the left. The positions of OSAs are indicated by arrows. (B) Penultimate threonine phosphorylation in H⁺-ATPase of rice. Rice seedlings at 5 d after germination were adapted to dark for >12 h prior to 10 μM FC (+) or mock (–) treatment. Top column, immunoblot using anti-CAT and anti-pTh. Bottom column, graphs quantifying the relative phosphorylation level (anti-pThr detection level normalized by the anti-CAT detection level). Mean ± SD (n = 3).