. 2016 Apr 14;28(5):1035–1052. doi: 10.1105/tpc.15.00703

Table 1. ProLRE:LRE-cYFP Restored the Reduction in Transmission of the lre-7 Mutation through the Female Gametophyte.

Female Parent^a	Male Parent^a	Observed No. of Progeny		TE (R/S)^b	χ²^c	P Value
Female Parent^a	Male Parent^a	Basta^R^d	Basta^S^d	TE (R/S)^b	χ²^c	P Value
Wild type	lre-7/+	180	164	1.10	0.740	0.388
lre-7/+	Wild type	22	194	0.11	137.000	<0.001
Line 23	Wild type	141	140	1.01^e	0.004	0.952
Wild type	Line 23	138	131	1.05	0.180	0.670

Line numbers refer to a ProLRE:LRE-cYFP transformant in the lre-7/lre-7 background containing a single insertion of the ProLRE:LRE-cYFP transgene. Genotype of the transgenic line used is homozygous for the transgene (ProLRE:LRE-cYFP/ProLRE:LRE-cYFP) and heterozygous for the lre-7 mutation (lre-7/+).

Transmission efficiency (TE) was calculated as the ratio of Basta resistance (R) to susceptibility (S) in the progeny of the indicated cross.

χ2 was calculated based on the expectation of a 1:1 segregation of Basta resistance to susceptibility in the progeny of a cross between the wild type and lre-7/+.

Basta-resistant (Basta^R) and Basta-susceptible (Basta^S) progeny. Basta resistance gene is linked with the T-DNA that is inserted into the LRE gene in lre-7 mutant.

When segregation ratio in row three was compared to that in row two as the expected segregation ratio, the χ² = 491.31 and P value < 0.001.