Table 2. ProPLL3:LRE-cYFP in the Male Gametophyte Restored the Reduction in Transmission of the lre-7 Mutation through the Female Gametophyte.
| Female Parent |
Male Parenta |
Observed No. of Progeny |
TE (R/S)b |
χ2c |
P Value |
|
|---|---|---|---|---|---|---|
| Resistantd | Susceptibled | |||||
| Wild type | lre-7/+ | 405 | 397 | 1.02 | – | – |
| lre-7/+ | Wild type | 100 | 542 | 0.18 | – | – |
| lre-7/+ | Line 3 | 150 | 215 | 0.70 | 180.77 | <0.001 |
| lre-7/+ | Line 5 | 176 | 285 | 0.62 | 179.09 | <0.001 |
| lre-7/+ | Line 6 | 226 | 316 | 0.72 | 281.24 | <0.001 |
| Wild type | lre-5/+ | 435 | 414 | 1.05 | – | – |
| lre-5/+ | Wild type | 53 | 448 | 0.12 | – | – |
| lre-5/+ | Line 3 | 94 | 130 | 0.72 | 233.25 | <0.001 |
| lre-5/+ | Line 5 | 203 | 328 | 0.62 | 429.17 | <0.001 |
| lre-5/+ | Line 6 | 158 | 264 | 0.60 | 321.88 | <0.001 |
Line numbers refer to three independent transformants in the wild-type background containing a single insertion of the ProPLL3:LRE-cYFP transgene; genotype of each transgenic line used is homozygous for the transgene (ProPLL3:LRE-cYFP / ProPLL3:LRE-cYFP).
Transmission efficiency (TE) was calculated as the ratio of Basta or kanamycin resistance (R) to susceptibility (S) in the progeny of the indicated cross.
χ2 was calculated based on Basta or kanamycin resistance to susceptibility segregation ratio (R:S) in the progeny of the crosses lre-7/+(♀) × wild type(♂) or lre-5/+(♀) × wild type(♂), respectively, as the expected segregation ratio.
Resistant and susceptible progeny to Basta or kanamycin. Basta and kanamycin resistance genes are linked to the T-DNA inserted in the LRE gene in lre-7 and lre-5, respectively.