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. 2016 Apr 19;28(5):1053–1077. doi: 10.1105/tpc.15.00776

Figure 12.

Figure 12.

NIP4;1 and NIP4;2 Transport Water and Glycerol in X. laevis Oocytes.

Water ([A] and [B]) and glycerol (C) transport assays in X. laevis oocytes. Permeability measurements of oocytes injected with EGFP-NIP4;1, EGFP-NIP4;1-S267A, EGFP-NIP4;2, and EGFP-NIP4;2-S267A cRNA and coinjections. The amount of cRNA (ng) is shown in parentheses. F. ananassa PIP2;1, Arabidopsis NIP6;1, and E. coli GlpF were used as positive controls for water and glycerol transport. Noninjected (NI) oocytes were used as negative controls. Representative result of three independent experiments is shown. Shown are the mean permeability values for water (Pf) and glycerol (Pg) ± se, n = 6 to 12. Different letters indicate significant differences among treatments (P < 0.05, one-way ANOVA, Tukey test).