Figure 13.

Functional Assays of NIP4;1 and Mutant NIP4;1-S267A in Yeast.

(A) Yeast growth and survival on synthetic medium with different concentrations of boric acid. Yeast strain ∆dur3 transformed with the empty vector pYeDP60u or pYeDP60u containing the indicated AQP homologs were spotted at an OD₆₀₀ of 0.02 and 2 on medium containing various concentrations of boric acid (0, 5, 10, 12.5, 15, 20, and 25 mM). Growth was recorded after 6 d at 30°C. All data were repeated in three independent experiments.

(B) Yeast growth and survival on synthetic medium with different concentrations of ammonia. The ∆mep1-3 yeast mutant transformed with the empty vector pYeDP60u or pYeDP60u containing the indicated AQP homologs was spotted at an OD₆₀₀ of 2, 0.02, or 0.0002 on medium containing various concentrations of ammonia (2, 3, 4, 5, and 10 mM) or 0.2% proline as sole nitrogen source and growth was recorded after 9 d at 30°C. All data were repeated in three independent experiments.