Figure 1.

Induction of Extracellular Thiocyanate after MPK3/MPK6 Activation or B. cinerea Infection Is Dependent on IGS Biosynthetic Pathway.

(A) Activation of MPK3/MPK6 in the gain-of-function GVG-Nt-MEK2^DD transgenic plants (DD) seedlings after DEX treatment induces the accumulation of thiocyanate in culture medium. Twelve-day-old DD, DD mpk3, and DD mpk6 seedlings were treated with DEX (1 μM). FW, fresh weight.

(B) B. cinerea-induced accumulation of thiocyanate is dependent on CYP79B2/CYP79B3 and other components in the IGS biosynthesis pathway. Twelve-day-old seedlings were inoculated with B. cinerea (Bc) spores (4.0 × 10⁵ spores per vial). Medium samples were collected at indicated times. The levels of thiocyanate ion in culture medium were quantified using the isonicotinic-barbituric acid method. Error bars indicate sd (n = 3). Two-way ANOVA was performed to compare the levels of thiocyanate ions in different genotypes after treatment. Lowercase letters above the columns indicate statistically different groups (P < 0.05).