ERF6, a Substrate of MPK3/MPK6, Plays an Important Role in the B. cinerea-Induced Biosynthesis of 4MI3G from I3G.
(A) Steady state levels of I3G and 4MI3G in 12-d-old Col-0, 35S:ERF6WT, 35S:ERF64D, and 35S:ERF6-EAR seedlings. One-way ANOVA was used to compare the levels of IGSs in different genotypes (P < 0.05).
(B) Intracellular levels of I3G and 4MI3G in 12-d-old Col-0 and 35S:ERF6-EAR seedlings after B. cinerea (Bc) inoculation.
Samples were collected without treatment (A) or at indicated times after treatment (B), and IGS levels were determined by HPLC assay. IGS contents in different genotypes were analyzed by two-way ANOVA. Different lowercase letters above the columns indicate statistically different groups (P < 0.01). Error bars indicate sd (n = 3). ns, not significant.