I3G Biosynthesis Is Highly Induced in Response to B. cinerea Infection or MPK3/MPK6 Activation.
(A) Steady state levels of I3G and 4MI3G in 12-d-old Col-0, cyp79b2/b3, and cyp81f2 seedlings after inoculation with B. cinerea (Bc) spores.
(B) Steady state levels of I3G and 4MI3G in 12-d-old DD, DD cyp81f2 seedlings after DEX treatment. Samples were collected at indicated times after treatment, and IGS levels were determined by HPLC assay. Error bars indicate sd (n = 3). Differences in IGS contents between different genotypes were analyzed by two-way ANOVA. Different lowercase letters above the columns indicate statistically different groups (P < 0.01).