Figure 4.

Cell Wall Analyses of tar2l^hypo and ein2l1^hypo Root Segments Compared with Their Wild-Type Backgrounds.

(A) Glycosidic linkage analysis of Bd21-0 and tar2l^hypo root segments.

(B) Glycosidic linkage analysis of Bd21-3 and ein2l1^hypo root segments.

(C) Neutral sugar analysis of Bd21-0 and tar2l^hypo root segments.

(D) Neutral sugar analysis of Bd21-3 and ein2l1^hypo root segments.

(E) Yariv staining (brownish) against β-1,3-galactan linkages in AGPs on longitudinal sections of Bd21-0 and tar2l^hypo root tips.

(F) 2F4 antibody staining against demethylesterified pectin (green) on transverse sections from the elongation zone of Bd21-0 and tar2l^hypo root tips.

(G) JIM7 antibody staining against methylesterified pectin (red) on transverse sections from the elongation zone of Bd21-0 and tar2l^hypo root tips.

(H) LM2 antibody staining against AGP side chains (red) on transverse sections from the elongation zone of Bd21-0 and tar2l^hypo root tips. Error bars indicate se of the mean (two technical replicates per each of three biological replicates). Differences were not statistically significant (Student’s t test) unless indicated as follows: *P < 0.05, **P < 0.01, and ***P < 0.001.