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. 2016 Apr 20;28(5):1200–1214. doi: 10.1105/tpc.16.00007

Figure 5.

Figure 5.

UBP14 Physically Interacts with UVI4.

(A) UBP14 physically interacts with UVI4 in vitro. MBP-UBP14 was pulled down (PD) by GST, GST-CCS52A1, or GST-UVI4 immobilized on GST beads and analyzed by immunoblotting (IB) using an anti-MBP antibody. IN represents input.

(B) UBP14 interacts with UVI4 in vivo. N. benthamiana leaves were transformed by injection of Agrobacterium GV3101 cells harboring Pro35S:Flag-UVI4 and Pro35S:GFP, Pro35S:GFP-CCS52A1, or Pro35S:GFP-UBP14 plasmids. Total proteins were immunoprecipitated (IP) with GFP-Trap-A, and the immunoblot was probed with anti-GFP and anti-Flag antibodies, respectively.

(C) UBP14 associates with UVI4 in Arabidopsis. Total proteins from Pro35S:Myc-CCS52A1 or Pro35S:Myc-UVI4 seedlings were immunoprecipitated with anti-Myc-Tag mouse mAb conjugated agarose beads, and the immunoblot was probed with anti-UBP14 and anti-Myc antibodies, respectively.

(D) UBP14 associates with UVI4 in Arabidopsis. Total proteins from Pro35S:GFP or ProUVI4:UVI4-GFP seedlings were immunoprecipitated with GFP-Trap-A, and the immunoblot was probed with anti-UBP14 and anti-GFP antibodies, respectively.