Figure 4.

Effects of TFIIIA on PSTVd Accumulation in N. benthamiana Plants.

(A) Downregulating expression of TFIIIA resulted in reduced accumulation of PSTVd as well as 5S rRNA, shown by RNA gel blotting. Immunoblots of leaf extracts showed reduced levels of TFIIIA-7ZF as well as increased level of TFIIIA-9ZF upon antisense suppression of TFIIIA. Histone H3 served as a loading control for protein gel blots, while ethidium bromide staining of rRNAs served as a loading control for RNA gel blots.

(B) Increased replication of PSTVd upon ectopic expression of TFIIIA-7ZF but not of TFIIIA-9ZF.

The quantification and normalization for the experiments are shown on the right of each gel panel. Gel loading controls were used as a standard for normalization. The band intensities were quantitated and normalized to the corresponding standards to obtain their percentage or fold values. Three biological repeats were used for the statistical analyses (**P < 0.01 and *P < 0.05). c, circular PSTVd, l, linear PSTVd.