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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Lipids. 2016 May 31;51(7):887–896. doi: 10.1007/s11745-016-4164-7

Fig. 1.

Fig. 1

Comparison of EtnGpl species present in rat myocardial extracts prepared with different buffer conditions and analyzed after different storage periods. Lipid extracts were prepared from rat heart with the BUME method in the presence of acetic acid or LiCl in the aqueous phase as described under the section of “Materials and Methods”. The lipid extracts were derivatized with Fmoc chloride prior to the analysis and EtnGpl species present in the derivatized lipid extracts were detected by neutral loss scan MS spectra of 222.2 Da (NLS222.2) as described under the section of “Materials and Methods”. Samples prepared with acetic acid (Panels A and C) and LiCl (Panels B and D) were analyzed on the day of extraction (Panels A and B) or after being stored for 48 days (Panels C and D). The MS spectra of NLS222.2 were acquired at collision energy of 26 eV and collision gas pressure of 1 mTorr.