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. Author manuscript; available in PMC: 2016 Jun 13.
Published in final edited form as: Mol Cell. 2015 Feb 26;57(6):1011–1021. doi: 10.1016/j.molcel.2015.01.026

Figure 2. Mechanism of Generation of Toxic MBP-1 From Non-Toxic Cores.

Figure 2

(A) BKA. Granule cores were resuspended in PBS at the indicated pH values to mimic the acidification process of the granules during eosinophil activation. Incubating the cores at pH 4 resulted in a significant rescue of toxicity in one trial. Values (mean levels ± % SD of one representative experiment, n = 3) are normalized to the buffer control.

(B) As measured by A280, twice as much soluble protein was released from nanocrystals at pH 3 compared with pH 7.3 (left panel). On the right, BKA performed with protein released from the cores at pH 3 and 7 upon normalization by concentration.

(C) BKA. The material released from the cores at pH 3 was either unfiltered or filtered through a 100 nm filter. Both samples had similar toxicities. Values (mean levels ± % SD of a representative experiment) are normalized to the buffer control.