Figure 2. No genotype differences were found in early developmental milestones (Bethesda cohort) and separation-induced pup ultrasonic vocalizations (Paris cohort) in Nlgn4 mice.

Analysis of markers of developmental milestones revealed no genotype differences in wild-type (+/+), heterozygous Nlgn4^+/− (+/−) and null mutant Nlgn4^−/− (−/−) pups between age day 2 and day 14, on measures of (a) body weight, (b) body length, (c) righting reflex and (d) eye opening. +/+, N =8; +/−, N =18; −/−, N =17. (e) Number of ultrasonic vocalizations emitted by pups separated from the nest did not differ significantly among genotypes. +/+, N =15–17; +/−, N =25–31; −/−, N =20. (f) Call repertoires were similar among genotypes on age day 8. +/+, N =11; +/−, N =18; −/−, N =14. (g) Spectrograms of the 11 call types analyzed. Numeric labels: 1=short; 2=flat; 3=upward; 4=downward; 5=modulated; 6=complex; 7=one frequency jump; 8=frequency jumps; 9=mixed; 10=unstructured; 11=others. Data are presented as mean±SEM in this figure and all other figures.