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. Author manuscript; available in PMC: 2016 Jun 13.
Published in final edited form as: Cell Host Microbe. 2016 May 11;19(5):686–695. doi: 10.1016/j.chom.2016.04.005

Figure 6. Viral Downregulation of HLA-C Impairs T Cell Inhibition of Viral Replication In Vitro.

Figure 6

(A and B) Primary CD4+ cells expressing HLA-C* 03:04 were infected in vitro with the HIV-1 molecular clones NL4-3 and AD8 (A) or NL4-3 and chimera 5, the mutant of NL4-3 containing a 149-bp segment substituted from WITO (B). Viral replication quantified over the course of 8 days is shown for CD4 cells cultured alone (black), cocultured with an Env-specific HLA-C*03-restricted CTL clone at a CTL:CD4 ratio of 1:4 (red) and uninfected cultures (gray). Results are representative of CTL:CD4 ratios from 1:1 to 1:20 and four replicate experiments using multiple CD4 donors.

(C) Flow cytometry staining of HLA-C on infected (red) or uninfected cells (black) and an isotype control (gray) is shown for each of the above experiments at day 6 post-infection, with infected cells in a culture discriminated by co-staining the viral protein Gag.

See also Figure S4.