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. 2014 Dec 23;1(3):e964037. doi: 10.4161/23723548.2014.964037

Table 1.

Cohort characteristics and detection approaches for key regulators of apoptosis execution in previous studies

References Cohorts Clinicopathologic data and detailed staging information Analytical methods Probes/primers/antibodies
Apaf-1        
Soengas et al.33 6 PMs Unknown gDNA: PCR Microsatellite marker analysis on 12q22–23: D12S393
  24 MMs   mRNA: ISH Riboprobes targeting fragments: 1–461, 581–1363, 2112–2225, 3426–3710
      mRNA: NB cDNA fragments to nucleotides 272–2908 and 1517–3525
      protein: IB mAb 6–1–19, pAb 8.46
Fujimoto et al.34 62 PMs PMs/MMs: Stage, thickness gDNA: PCR Microsatellite marker analysis on 12q22–23: D12S1657, D12S393, D12S1706, D12S346
  112 MMs   mRNA: PCR Forward 5’-ACATTTC TCACGATGCTACC-3’; reverse 5’-CAATTCATGAAGTGGCAA-3’
Fujimoto et al.35 49 MMs Stage gDNA: PCR Microsatellite marker analysis on 12q22–23: D12S1657, D12S393, D12S1706, D12S346
Niedojadlo et al.36 75 nevi PMs: Clark levels, thickness mRNA: FISH RNA probes prepared from a plasmid provided by Dr. Xang, University of Texas, USA
  53 PMs   protein: IHC Rat mAb H19, provided by Dr. O'Reilly, WEHI, Melbourne, Australia
  24 MMs      
Baldi et al.37 30 nevi PMs: Thickness protein: IHC Rabbit pAb, H-324, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA
  61 PMs      
  15 MMs      
Mustika et al.38 10 nevi PMs: Thickness protein: IHC mAb, Santa Cruz Biotechnology Inc.
  11 in situ melanomas      
  26 PMs      
  15 MMs      
Dai et al.39 13 nevi PMs: Thickness protein: IHC Rabbit pAb, BD Biosciences, Mississauga, Ontario, Canada
  70 PMs      
Zanon et al.40 8 nevi Unknown protein: FC mAb from Chemicon International, Temecula, CA, USA
  16 PMs   protein: IHC Rat mAb from Chemicon International
  71 MMs      
Bachmann et al.41 31 nevi PMs: Thickness protein: IHC pAb RB 9236, Neomarkers
  153 PMs      
Caspases        
Woenckhaus et al.49 16 nevi PMs: Thickness protein: IHC Cleaved caspase-6, New England Biolabs, Frankfurt, Germany
  20 PMs     Cleaved caspase-3, New England Biolabs
  30 MMs      
Chen et al.42 24 nevi Cutaneous PMs: staging protein: IHC Caspase-3: rabbit pAb, Santa Cruz Biotechnology, Inc.
  52 PMs - cutaneous Mucosal PMs: staging   Caspase-6: goat pAb, Santa Cruz Biotechnology, Inc.
  25 PMs - mucosal     Caspase-7: goat pAb, Santa Cruz Biotechnology, Inc.
Liu et al.47 57 PMs Thickness protein: IHC Rabbit anti-active caspase-3, Abcam, Cambridge, UK
XIAP        
Kluger et al.54 540 nevi Unknown protein: IHC Mouse Ab, BD Transduction Laboratories
  232 PMs      
  299 MMs      
Emanuel et al.53 6 nevi PMs: Thickness protein: IHC Mouse mAb, clone 48, BD Biosciences, San Jose, CA, USA
  4 in situ melanomas      
  67 PMs      
Chen et al.42 24 nevi Cutaneous PMs: staging protein: IHC Rabbit pAb, Santa Cruz Biotechnology Inc.
  52 PMs - cutaneous Mucosal PMs: staging    
  25 PMs - mucosal      
Hiscutt et al.55 6 nevi Staging and thickness protein: IHC mAb, BD Biosciences, Oxford, UK
  7 in situ melanomas      
  42 PMs      
ML-IAP        
Nachmias et al.59 27 PMs Unknown protein: IB Mouse mAb, clone 88C570, Imgenex
Gong et al.57 14 nevi Unknown mRNA: ISH antisense 5'-CAAAGACGATGGACACGGC-3'; sense 5'-GCCGTGTCCATCGTCTTTG-3'
  19 PMs (cut/muc)   mRNA: RT-PCR upstream 5'-ATGGGCTCTGAGGAGTTGCGTC-3';
  15 MMs     downstream 5'-CATAGCAGAAGAAGCACCTCACCTTG-3'
      protein: IHC Rabbit pAb, R&D Systems, Minneapolis, MN, USA
Takeuchi et al.58 63 MMs Staging mRNA: qRT-PCR FRET probe: 5'-FAM-TGAGCTGCCCACACCCAGGAGAG-BHQ-1-3'
Chen et al.42 24 nevi Cutaneous PMs: staging protein: IHC Goat pAb, Santa Cruz Biotechnology Inc.
  52 PMs - cutaneous Mucosal PMs: staging    
  25 PMs - mucosal      
Apollon        
Tassi et al.61 8 PMs (cell lines) Unknown protein: FC, IB Mouse Ab, BD Biosciences
  26 MMs (cell lines)      
c-IAPs        
Chen et al.42 24 nevi Cutaneous PMs: staging protein: IHC c-IAP1: rabbit pAb, Santa Cruz Biotechnology Inc.
  52 PMs - cutaneous Mucosal PMs: staging   c-IAP2: rabbit pAb, Santa Cruz Biotechnology Inc.
  25 PMs - mucosal      
XAF1        
Ng et al.65 40 nevi PMs: Thickness protein: IHC Rabbit Ab provided by Dr. Korneluk, University of Ottawa, Canada
  70 PMs      

Abbreviations: Cut, cutaneous; FC, flow cytometry; FISH, fluorescence in situ hybridization; IB, immunoblot; IHC, immunohistochemistry; ISH, in situ hybridization; MM, metastatic melanoma; muc, mucosal; NB, Northern blot; PCR, polymerase chain reaction; PM, primary melanoma; qRT-PCR, quantitative real time polymerase chain reaction.