Figure 3. Leukemia inhibitory factor (LIF) treatment induces de-differentiation phenotype of nasopharyngeal carcinoma (NPC) cells. (A) Treatment with LIF or soluble LIFR (sLIFR) induces morphologic changes in NPC-TW06 cells. Upper panels, bright field; lower panels, fluorescent staining of actin (red) and DAPI (blue). (B) mRNA expression of genes associated with epithelial differentiation by QRT-PCR. Values are presented as means ± SD of duplicate samples.