Figure 2.

Proteins that were specifically enriched in the pull-down experiments. (A) and (B) show the two independent experimental replicates of the pull-down experiments with each single experiment performed in triplicate. The average values of the label free quantification (LFQ) with their standard deviations are indicated in the graphs for all proteins that were significantly enriched in at least one triplicate (red: elution with 500 µM GTP; orange: control experiment not containing immobilized Sec4 and elution with 500 µM GTP; blue: elution with 10 mM GTP; cyan: control experiment not containing immobilized Sec4 and elution with 10 mM GTP; * indicates statistically relevant enrichment comparing the pull-down and the control experiment (t-test)). The insets show the same graphs with logarithmic scale of the LFQ intensities for better visualization of the differences between the experiments and the corresponding controls.