Figure 5.

Putative phosphatidylserine (PS) binding motif in Rabin8. (top) Schematics of the domain architecture of Rabin8 with numbering from the human sequence. (bottom) multiple sequence alignment of the flexible linker region (reside 250–290) of Rabin8 located between the Rab8 GEF domain and the Rab11-binding domain. Serine 272 that is phosphorylated by NDR-2 is colored magenta and the positively charged residues that form a potential PS-binding motif are colored blue. Consensus motif of NDR substrates (HxRxxS/T) is absent from Sec2, nevertheless S186 and S188 in the linker region are also phosphorylated by another kinase.⁸⁰ Clustal2w was used for the alignment. Abbreviations used are: Hs: Homo sapiens, Bt: Bos Taurus, Mm: Mus musculus, Mv:Manacus vitellinus, Xt: Xenopus tropicalis, Dr: Danio rerio, Sc: Saccharomyces cerevisiae.