Table 2.
Lysine-residues in Ras-proteins representing the 5 major Ras-subfamilies identified to be lysine-acetylated by quantitative proteomics as found in PhosphoSitePlus.49 The sites in human were found in A549 (pulmonary), HeLa (cervical), Jurkat (T lymphocyte), K562 (erythroid) and/or MV4-11 (macrophage) cells.33,46,50 The sites in mouse were identified in liver tissue and in the ones in rat were found in 11 different tissues (brain, heart, kidney, liver, lung, pancreas, skin, spleen, stomach, testis, thymus)5
| Lysine-acetylation sites found by MS |
|||
|---|---|---|---|
| GNBP | Human | Mouse | Rat |
| Ran | K37,60,71,99, 152,159 | K60,71,159 | K23,28,37,60,99, 134,152,159 |
| K-Ras | K104 | — | — |
| N-Ras | — | — | — |
| H-Ras | — | — | — |
| Rap1A | — | — | K151 |
| Rap1B | — | — | K151 |
| RhoA | — | — | K133,135 |
| RhoB | — | — | — |
| RhoC | — | — | — |
| Cdc42 | K135,144,153 | — | K128,133,135, 144,150,153,166 |
| Rac1 | — | K132, K133 | K147,153 |
| Rab1A | K61 | — | K61,132,140 |
| Rab1B | K58 | — | — |
| Rab7A | — | — | K32 |
| Rab7B | — | — | — |
| Rab7L | — | — | — |
| Arl1 | — | — | K152 |
| Arf1 | K36,142 | K142 | K36,142 |