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. 2015 Aug 20;3(2):e1046579. doi: 10.1080/23723556.2015.1046579

Figure 2.

Figure 2.

Increased p62 turnover and GFP-LC3 and LTR puncta in PINK1−/− MEFs. (A) Higher magnification images showing immunofluorescence of mCherry-GFP-p62 (cells represented in the insets) on MEFs transiently transfected for 24 h and treated as described in Figure 1A. Arrows indicate the pattern of each condition. Scale bars: 10 μm. (B) Higher magnification images of immunofluorescence of GFP-LC3 and LTR (cells represented in the insets) on MEFs transiently transfected for 24 h, stained with LTR and treated as described in Figure 1A. Arrows indicate the pattern of each condition. Scale bars: 10 μm. (C) Percentage of mCherry-p62 (+) puncta per cell. (D) Number of GFP-LC3 puncta per cell. (E) Number of LTR puncta per cell.