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. 2015 Aug 20;3(2):e1046579. doi: 10.1080/23723556.2015.1046579

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Figure 3. — Ultrastructural mitophagic/autophagic hallmarks are upregulated in PINK1^−/− MEFs. (A) Higher magnifications views of the boxed regions are shown in insets. Scale bar: 10 μm. Mitochondria (M) and autophagic vacuoles (AVs) are shown in the enlarged view. Scale bar insets: 2 μm. (B–E) Analysis of mitochondrial parameters (number of mitochondria, 197 for PINK1^+/+ MEFs and 130 for PINK1^−/− MEFs) including area (B), perimeter (C), Feret's diameter (D) and circularity (E). (F) Representative western blot analysis of COX IV. Molecular mass is indicated in kilodaltons (kDa) next to the blots. (G) Graph showing relative amount of COX IV, in arbitrary units of intensity, determined by optical densitometry. Data were normalized to expression of α-tubulin. (H) Number of AVs per cross-sectioned cell. n = 25 (PINK1^+/+ MEFs); 8 (PINK1^+/+ MEFs + Baf. A1 + EBSS); 9 (PINK1^−/− MEFs); 7 (PINK1^−/− MEFs + EBSS); 8 (PINK1^−/− MEFs + Baf. A1); 6 (PINK1^−/− MEFs + Baf. A1 + EBSS). (I) Electron micrograph detail, showing different vesicles considered in this study as AVs.