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Transfection of PINK1−/− MEFs with PINK1 WT or kinase mutants reduces autophagy response to basal levels. (A) Representative immunofluorescence images of mCherry-GFP-LC3B and V5-tagged PINK1 (represented in the insets) in MEFs after transient cotransfection for 24 h. Arrows indicate the pattern of each condition. Scale bars: 10 μm. (B) Percentage of mCherry-LC3B (+) puncta per cell.
