Skip to main content
. 2016 Feb 13;7(10):10696–10709. doi: 10.18632/oncotarget.7372

Figure 1. RNAi synthetic lethal screen.

Figure 1

A. Schema of shRNA screen. The change in the relative abundance of each shRNA in the library over time is measured using the normalized PD 13/PD 0 ratio of its reads. A log2 PD 13/PD 0 ratio of < 0 indicates the shRNA is depleted in the population over time, and a log2 PD 13/PD 0 ratio of < 0 indicates the shRNA is enriched in the population. B. Hit kinase genes. Targets were filtered by log2 PD 13/PD 0 ratio of < −2 (n = 3). C. Factorial dose matrix combinatorial drug treatment. HN12 cells were incubated for 72 hrs with indicated concentrations of drugs. Numbers on the matrix indicate % Cell Viability (n = 3). D. Computer-simulated Fa-CI curves were created based on the matrix data. The ratios of rapamycin : trametinib were indicated. Synergism (CI < 1), additive effect (CI = 1), or antagonism (CI > 1) for the indicated levels of growth inhibition (Fraction affected) induced by the drug combination. E. mTOR/ERK pathway. HN12 cells were treated with 0.1% DMSO, 20nM rapamycin, 20nM trametinib or the combination for 24hrs.