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. 2016 Feb 21;7(10):10723–10738. doi: 10.18632/oncotarget.7561

Figure 7. Esophagus-specific functional relationship network among Fbxw7, Stk40, and Pdcd4 using the Functional Networks of Tissues in Mouse (FNTM) prediction tool.

Figure 7

A. The displayed esophagus-specific nine-gene network shows predicted functional relationships among the genes that are most functionally related to Stk40, Pdcd4 and Fbxw7 (tumor-suppressor targets of miR-31, mir-21 and miR-223, respectively). The edges between genes are colored by the confidence of the predicted relationship. B. Enrichment Gene Ontology analysis for the nine genes retrieved from FNTM analysis. Terms exceeding an FDR-corrected P-value of 0.01 are excluded. C. Quantitative polymerase chain reaction (qPCR) analysis of esophageal Pten expression in ZD3T and ZST esophagus (n = 6 rats/group; Oaz1 as normalizer, *P = 0.02). Error bars represent standard deviation. D. Immunohistochemical analysis of PTEN protein expression in ZD3T and ZST esophagus showing cytoplasmic PTEN expression in ZST esophageal epithelial cells (black arrow head, brown, 3,3′-diaminobenzidine tetrahydrochloride) and lack of PTEN expression in ZS3T esophageal ESCC tumor cells Stromal cells and blood vessels showed intensely positive expression for PTEN (red arrow head, served as integral internal positive). ZD3T and ZST = marked-ZD and Zn-sufficient tumor groups. Scale bars = 25 μm, x400 magnification.