Figure 1. Maf1/MAFR-1 is required for calorie restriction to extend life in yeast but not C. elegans.

A. Maf1 is required for calorie restriction (CR) to extend lifespan in S. cerevisiae. WT and maf1Δ cells were cultured normally (2% glucose) or calorie restricted (0.2% glucose) to stationary phase and fractions of survival at indicated time points were measured by colony forming assay. Error bars stand for the standard error of the mean (SEM) of 3 independent experiments. Chi-square test: ***p < 0.0001, ns, not significant. B. Maf1 is required for TOR1 deletion to extend lifespan in S. cerevisiae. WT, tor1Δ, maf1Δ and tor1Δ maf1Δ cells were cultured to stationary phase and the fractions of survival at indicated time points were measured by colony forming assay. Error bars stands for the SEM of 3 independent experiments. Chi-square test: ***p < 0.0001, ns, not significant. C. Maf1 homolog in C. elegans (MAFR-1) is not required for the calorie restriction model eat-2 mutant to live long. eat-2 mutation reduces animal's pharyngeal pumping therefore extending lifespan by calorie restriction. Animals were raised on OP-50 bacteria in plates containing FUDR to inhibit progeny from growth. Dead worms were recorded every other day and percentage of survival was plotted. Chi-square test: ***p < 0.0001, **p < 0.001. D. MAFR-1 is not required for food deprivation to extend lifespan. Animals were raised on OP-50 bacteria in plates containing FUDR to young adulthood and transferred to new plates containing no bacteria. Chi-square test: ***p < 0.0001, *p < 0.01. E. MAFR-1 is not required for bacterial dilution to extend lifespan. Animals were raised on control and diluted OP-50 bacteria plates and transferred to fresh plates every other day (See Supplemental Data for details). Chi-square test: **p < 0.001.