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A. Structures of the six lead quinazolines. B. Cancer cells in 6-well plates were treated with test compounds at 10 μM (HCT116) or 20 μM (SW480) for 24 h. The levels of axin2, cyclin D1 and survivin were examined by Western blotting. All the samples were also probed with anti-actin antibody to verify equal loading.
