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. 2016 Feb 8;7(10):11349–11363. doi: 10.18632/oncotarget.7239

Figure 4. IL-1R1 is up-regulated in gastric cancer tissues and it is the direct target of HNF4α.

Figure 4

A. The mRNA levels of IL-1R1 in 30 atrophic gastritis samples and 30 gastric cancers were measured by real-time PCR. The horizontal bar indicates the mean value of each sample group. Mann-Whitney U-test is used to calculate the P value. B. Positive correlation between HNF4α and IL-1R1 expression in the above tissues. Each data point represents an individual gastric tissue sample, and a correlation coefficient r is shown. C. RT-PCR indicates IL-R1 mRNA levels in indicated cells transfected with HNF4α siRNA and HNF4α over-expression plasmid. D. Luciferase reporter assay confirmed the direct interaction between HNF4α and IL-1R1 promoter. Data are mean±SD of 3 biological replicates. *p<0.05,**p<0.01,***p<0.001 by Student's t-test. E. and G. IL-R1 protein level changes in indicated cells transfected with HNF4α siRNA and HNF4α over-expression plasmid using western blot and immunofluorescence. F. Western blot shows HNF4αand IL-1R1 protein expression in tissues from nude mice in NC group and Hsh group.