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. 2016 Feb 5;7(10):11463–11477. doi: 10.18632/oncotarget.7201

Figure 2. RKIP reduction increases NPC cell radioresistance in vitro.

Figure 2

(A) a representative result of Western blotting shows the levels of RKIP expression in the RKIP OE CNE2-IR cells, RKIP KD CNE2 cells and their corresponding EV-transfected cells. (B) a clonogenic survival assay shows that radioresponse of RKIP OE CNE2-IR cells, RKIP KD CNE2 cells and their corresponding EV-transfected cells. (top) cells were irradiated with a range of 1–8Gy radiation doses, and colonies that formed after incubation of 12 days were stained with crystal violet and photographed; (bottom) dose survival curves were created by fitting surviving fractions to the linear quadratic equation. (C) CCK-8 assay shows that proliferation of RKIP OE CNE2-IR cells, RKIP KD CNE2 cells and their corresponding EV-transfected cells after 4Gy irradiation. Three experiments were done; Means, SDs, and statistical significance are denoted; *P < 0.01. OE, overexpression; KD, knockdown; EV, empty vector.