Figure 4. LINC00857 regulates cell cycle at the G1/S phase and potentially through CCNE1 and CDK2 regulation.

(A) Knockdown using LINC00857 siRNA caused G₁ arrest in both H1299 and H838 lung cells. (B–C) LINC00857 siRNA knockdown induced 136 down and 116 up-regulated genes in both H1299 and H838 cells. (D) CCNE1 was decreased after LINC00857 siRNA treatment measured by both microarray and qRT-PCR for mRNA, and protein by Western blot. (E–F) The LINC00857 positively correlated genes in primary tumors were significantly involved in cell cycle regulation analyzed by gene ontology from DAVID.