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. 2016 Feb 9;7(10):11567–11579. doi: 10.18632/oncotarget.7272

Figure 3. HIF-3α1 can activates hypoxia response gene in CRC cells and is majorly located in the cytosol when stabilized.

Figure 3

(A) Enolase promoter (P2.1) luciferase assay in HIF-3α1-overexpressing or EV lentivirus infected HT29 and SW480 CRC cells. Cells were transfected with HIF-2α or EV plasmids. (B) Western blot analysis in the cytosolic and nuclear fraction from HIF-3α1-overexpressing or EV lentivirus infected SW480 CRC cells or colorectal tissues of VhlF/F and VhlΔIE mice. *p < 0.05, ***p < 0.001 compared with EV control cell line. ##p < 0.01, ###p < 0.001 compared with EV control plasmids. M.W., molecular weight.