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. 2016 Feb 9;7(10):11567–11579. doi: 10.18632/oncotarget.7272

Figure 4. Overexpression of HIF-3α1 activates STAT3 signaling in CRC cells.

Figure 4

(A) Western blot analysis in whole cell extracts from HIF-3α1-overexpressing or EV lentivirus-infected HT29 or SW480 CRC cells. (B) STAT3 activity luciferase assay in HIF-3α1-overexpressing or EV lentivirus infected HT29 and SW480 CRC cells. Cells were treated with or without IL6 (10 ng/mL) for 24 hours. (C) qPCR analysis of SOCS3 gene expression in HIF-3α1-overexpressing or EV lentivirus infected HT29 and SW480 CRC cells. *p < 0.05, **p < 0.01, ***p < 0.001 compared with EV control cell line. ##p < 0.01, ###p < 0.001 compared with untreated controls.