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. 2016 Feb 9;7(10):11595–11608. doi: 10.18632/oncotarget.7292

Figure 5. miR-150 inhibits HCC cell proliferation, migration and invasion by targeting GAB1.

Figure 5

A. Quantitation of GAB1 mRNA levels in SMMC-7721 cells by qRT-PCR after transfection with 3 different siRNAs targeting GAB1 (si-GAB1-1, si-GAB1-2 and si-GAB1-3) or a negative control siRNA (si-NC). B. Detection of GAB1 protein in SMMC-7721 cells by western blot analysis after transfection with GAB1 siRNAs or si-NC. C-E. GAB1 knockdown inhibited SMMC-7721 cell proliferation, colony formation, migration and invasion as demonstrated by CCK-8 proliferation assay (C), colony formation assay (D) and Transwell assay (E). F-H. GAB1 reintroduction into SMMC-7721 cells partially rescued the miR-150-mediated inhibition of cell proliferation, colony formation, migration and invasion as determined by CCK-8 proliferation assay (F), colony formation assay (G) and Transwell assay (H). Migrated and invaded cells were counted in 5 randomly selected areas under a 200× microscope field. *P<0.05.