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. 2016 Jan 30;7(10):11696–11707. doi: 10.18632/oncotarget.7077

Figure 6. LINC01133 function as oncogene by repressing KLF2 expression in NSCLC cells.

Figure 6

A549 cells were transfected with pCDNA-KLF2, or co-transfected with si-LINC01133 and si-KLF2. A. The protein level of KLF2 was detected by western blot. B, C, D. MTT assays, colony-forming and EdU staining assays were used to determine the cell viability. Values represent the mean ± s.d. from three independent experiments. E. Apoptosis was determined by flow cytometry. F. The protein level of KLF2 was detected by western blot. G. MTT assays, colony-forming and Edu staining assays were used to determine the cell viability. H. Analysis of the relationship between LINC01133 expression and KLF, P21 or E-cadherin expression levels. *P <0.05 and **P <0.01.