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. 2016 Feb 13;7(10):11733–11743. doi: 10.18632/oncotarget.7367

Figure 2. MALAT1 enhances AKAP-9 expression by promoting SRPK1-mediated SRSF1 protein phospholation.

Figure 2

(A) Immunostaining of phospholated SRSF1 protein in Scramble-SW480 RNAa-MALAT1-SW480 and RNAi-MALAT1-SW480 cells. Magnification: 1800x. (B) The phospholated SRSF1 protein in nucleus was detected by Western blot analysis using mAb1H4 antibody.(C) The phospholated SRSF1 protein levels were normalized to Histone. *P < 0.001 compared to the control group(Scramble), n = 3. (D) Immunostaining of SRPK1 protein in Scramble-SW480 RNAa-MALAT1-SW480 and RNAi-MALAT1-SW480 cells. Magnification: 1800x. (E) Western blot analysis of SRPK1 protein expression in Scramble-SW480 RNAa-MALAT1-SW480 and RNAi-MALAT1-SW480 cells. (F) The protein levels were normalized to α-Tubulin. *P < 0.05 compared to the control group (Scramble), n = 3. (G) RNAa-MALAT1-SW480 cells were treated with SRPK1 inhibitor SRPIN340 in different dose. (H) The protein levels were normalized to α-Tubulin. *P < 0.001 compared to the control group (Blank and dimethylsulphoxide 0.02%), n = 3.