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. 2016 Feb 13;7(10):11733–11743. doi: 10.18632/oncotarget.7367

Figure 3. Overexpression of SRPK1 in MALAT1-deficient SW480 cells (RNAi-MALAT1) restored AKAP-9 expression by reversing the level of SRSF1 phosphorylation.

Figure 3

(A) Overexpression of SRPK1 in RNAi-MALAT1 SW480 cells restored phospholated SRSF1 and AKAP-9 protein expression. (B) The phospholated SRSF1 and AKAP-9 protein levels were normalized to α-Tubulin, *P < 0.05 compared to the control group (Scramle-MALAT1), **P < 0.01 compared to the control group (RNAi-MALAT1/Vector), n = 3. (C) The phospholated SRSF1 protein in nucleus and cytoplasm were detected by Western blot analysis using mAb1H4 antibody respectively. The phospholated SRSF1 protein is enriched in nucleus. (D) The phospholated SRSF1 protein levels were normalized to Histone. *P < 0.05 compared to the control group (Scramle-MALAT1), **P < 0.01 compared to the control group (RNAi-MALAT1/Vector), n = 3.