Figure 1.

Schematic representations of the different Tobacco etch virus genotypes used in this study. The wild-type TEV (A) codes for 11 mature peptides, including P3N-PIPO embedded within the P3 protein at a +1 frameshift. Relevant names of the proteins products are indicated in the gray dashed boxes. For more information on the features and proposed functions of the proteins, consult Revers and García (2015). For constructing the viral genotypes with the NIb replicase in an alternative position, a copy of NIb was moved to the first and second positions within the TEV genome, removing NIb from its original position (B). For constructing the viral genotypes with a duplication of NIb, a second copy of NIb was introduced into the first and second positions within the TEV genome, preserving the original NIb and thereby generating a duplication event (C). For simplification, P3N-PIPO is drawn only at the wild-type TEV.