Figure 3.

The growth defect of disome IX cells lacking EDE1 is due to excess PRK1. (A) Cells were incubated with Lucifer yellow dye for 1 hr at room temperature, washed three times, and imaged. The distribution of internalization phenotypes is shown. n > 100 cells per strain. Images on the right show the different classes of Lucifer yellow localization: (a) vacuolar accumulation, (b) mixed vacuolar and membrane localization, and (c) membrane or no localization. (B and C) Doubling times were determined as described in Figure 2 in YPD medium (B) or synthetic complete medium without tryptophan (C). SD is shown. *P < 0.01, ****P < 0.00001; Student’s t-test.